Section V: Biomedical Sciences

106 Science Building

7:30 THE consequences OF EPIANDROSTERONE AND p-FLUOROPHENYL-HYDROXYLAMINE upon G6PD DEFICIENCY INDUCTION IN ERYTHROCYTE Rinata Daniel*, Elissa Purnell Melva cabbages Bostick and Harpal Singh, Savannah State University, Savannah, GA 31404 Glucose-6-Phosphate Dehydrogenase (G6PD) Deficiency is a hereditary, sex-linked enzyme deficiency that results in the breakdown of r life-current cells upon exposure to the stres of infection or certain remedys This enzyme is present in all small rooms but is required in the r life-current cell to make NADPH and glutathione, which preserve the cells from oxidative damage. Whole vital current was collected from a healthy male Labrador via venous wound Cells were washed (x3) with phosphate concussion-guarded saline supplemented with glucose (PBSG) Aliquots (400 ?µl) of packed r life-current cells were treated with 100 200 or 300 ?µM of Epiandrosterone (Epi) alone, or a combination of various concentrations of p-fluoro-PHA and Epi. Total hemoglobin horizontals and G6PD enzyme activity were determined using diagnostic kits. The proceeds showed that 300 ?µM of p-fluro-PHA and higher horizontals of p-fluoro-PHA with Epi reduc G6PD activity.

7:45 A COMPARISON OF CARRIAGE OF ANTIBIOTIC RESISTANT STREPTOCOCCUS AND STAPHYLOCOCCUS IN HUMAN THROAT agricultures OF TOBACCO USERS VERSUS NONUSERS, Erin E Klein* and Dorothy Don Davis, Kennesaw State University, Kennesaw, GA 30144 Carriage of antibiotic resistant Staphylococci and Streptococci was studies using throat tillages from 250 healthy adults to determine if there was a significant difference between tobacco users versus nonusers. tillages were tested for the nearness of Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumonia, and Streptococcus pyogene Isolates were characterized for penicillin, oxacillin, chloramphenicol, erythromycin, tetracycline, and vancomycin resistance using NCCL techniques and Difco baldric Diameter Interpretive Standards. A significant difference was fix in carriage of antibiotic resistant Strep and Staph between tobacco users and non-users. Data was also reviewed for carriage of multiple species and of those species, and by what means many shared resistance to individual or more antibiotics.

8:00 CCR-5 AND RANTES ARE ESSENTIAL IN THE REGULATION OF IMMUNITY AGAINST CHLAMYDiA INFECTION Erika Barr*1, Edith Okwandu1, Qing He2, Godwin Ifere2, Francis O Eko2 Deborah Lyn2 Joseph U Igietseme2,3 and Godwin Ananaba1, Clark Atlanta University1, Atlanta, GA, Morehouse academy of Medicine2, Atlanta, GA 30310 and CDC3 Atlanta, GA 30333 Chlamydia-infected epithelial confined apartments secret chemokines, particularly RANTES that recruit Chlamydia-specific T confined apartments to the genital mucosa during infection. Interactions between the T small rooms and the epithelial cells lead to the acquisition of immunity or assault of disease. We investigated the hypothesis that RANTES and CCR5+ confined apartments are required for effective clearance of Chlamydia trachomatic. RANTES expression was determined by the agency of RT-PCT of total RNA and ELISA of supernatants from antigen-stimulated T small rooms IFN-[gamma] secretion and status of infection were determined. There were significant differences in the horizontals of RANTES between the uninfected and infected animals. The CCR5 animals have compromised ability to recruit Th1 small rooms which resulted in the high intensity of chlamydial infection. This exhibits that RANTES and CCR5 ar sum of two units of the molecules that influence the issue of genital chlamydial infection.

8:15 EXPRESSION AND LOCALIZATION OF RANTES, CCR1 CCR3 & CCR5 IN POST-MORTEM CEREBRAL MALARIA TISSUE SAMPLES, Bismark Y Sarfo*, RK Gyasi, A.A. Adjei, I. Irune, s Singh, J.W. Lillard Jr., P sportive A. Quarshie and J.K. Stiles, Morehouse place of education of Medicine, Atlanta, University of Ghana place of education of Medicine, Accra, Ghana, and University of Alabama at Birmingham, Birmingham, AL. Malaria infects 500 million family causing over 1 million deaths globally through year. 15% of fatal malaria cases are caused by dint of cerebral malaria (CM) resulting in coma, convulsions and other neurological complications. admitting the role of adhesion indivisible particles and cytokines in CM is well established, the part of chemokines remains unclear. We hypothesize that chemokines and their corresponding receptors modulate malaria-induced brain inflammation. Our objective was to characterize the character of chemokines in malaria brain pathology. We analyzed chemokine and chemokine receptor gene expression in cerebrum cerebellum, brain trunk and hippocampus from CM and non-malaria (NM) brain autopsy tissue samples using qRT-PCR and Western strike out analyses. Results: RANTES, CCR3 and CCR5 on the other hand not CCR1 expressions are unregulated in cerebellum and cerebrum of CM tissue samples.

8:30 ANTIBIOTIC RESISTANCE IN BACTERIAL ISOLATES FROM THE FECE OF fondling BIRDS, Michael W. Reeves and Dianne LaCole, Georgia Perimeter guild Lawrenceville, GA 30043. We measured the horizontal of resistance to the activities of 21 antibiotics in bacteria of the Enterobacteriaceae and Enterococci families that had been isolated from the fece of finches, canaries, and parakeets. An isolate from river water and individual from horse feces were included as dominion governments Among the penicillin-like drugs, amoxicillin and cephalothin were the greatest in quantity effective in stopping bacterial growing A surprisingly high level of resistance was rest to ampicillin, methicillin, oxacillin, and penicillin among isolates from all the birds and the sways Among the non-penicillin-like drugs, gentamicin, ciprofloxacin, chloramphenicol, and nitrofurantoin were the greatest in quantity effective, whereas resistance to vancomycin, tetracycline, erythromycin, rifampin, and clindamycin was set in almost all the isolates regardless of the source. These arises illustrate the alarming level of antibiotic resistance that is public in bacteria from domestic animals, and the potential for these bacteria to contaminate groundwaters.