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Methods for the detection of trace metabolites from biotransformation of polycyclic aromatic hydrocarbons by yeast.

ABSTRACT--The analysis of pollutants and their metabolites resulting from biotransformation is important to studies of bioremediation or toxicology of environmental toxicants. Gas chromatography-mass spectrometry (GC-MS) with solid-phase micro-extraction (SPME) has been used to determine the 9-hydroxyphenanthrene horizontals resulting from phenanthrene biotransformation by the agency of yeast. Candida tropicalis CP1-1, derived from a parent strain of Candida tropicalis (ATCC strain 96745) was grown in minimal media containing phenanthrene. Direct extraction of liquid media through SPME served to selectively preconcentrate the pollutants prior to GC-M analysis. Various derivatizing reagents, including dimethyldichlorosilane, trimethylchlorosilane, acetic anhydride, and trifluoroacetic anhydride, were evaluated for their ability to enhance the


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